Real Madrid midfielder, Isco has been diagnosed with a hamstring injury after several scans on Wednesday revealed muscle damage in the attacking his right leg.
The Spain international came off pitch in the 68th minute during their weekend meeting with Real Valladolid where they played 1-1 home draw.
The Spanish side have not confirmed the severity of the setback, but the 27-year-old is likely to miss Sunday’s visit to Villarreal.
“His recovery will continue to be monitored,” the club added in a statement.
Isco’s injury is another blow to Los Blancos boss Zinedine Zidane, who has been without Eden Hazard, Ferland Mendy and long-term absentee Marco Asensio during LaLiga’s opening two rounds.
Los Blancos have also lost James Rodriguez to a calf problem, while youngsters Rodrygo Goes and Brahim Diaz remain sidelined.
Isco might additionally be forced to sit out his country’s Euro 2020 qualifiers against Romania and Faroe Islands on September 5 and 8, respectively